15 applications and implications

fernando meneses

base3

about meabout me
1 principles and practices1 principles and practices
2 computer-aided design2 computer-aided design
3 computer-controlled cutting3 computer-controlled cutting
4 electronics production4 electronics production
5 3D scanning and printing5 3D scanning and printing
6 electronics design6 electronics design
7 embedded programming7 embedded programming
8 computer-controlled machining8 computer-controlled machining
9 molding and casting9 molding and casting
10 input devices10 input devices
11 output devices11 output devices
12 composites12 composites
13 networking and communications13 networking and communications
14 interface and application programming14 interface and application programming
15 applications and implications15 applications and implications
16 mechanical design, machine design16 mechanical design, machine design
17 invention, intellectual property, and income17 invention, intellectual property, and income
18 project development18 project development
19 project presentation19 project presentation

 15_applications and implications, toward the final project:

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Open RGBacteria

what will it do?

It is an open and low cost device to identify bacteria .

State of the art

who’s done what beforehand?

In microbiology there are various techniques and technologies for the identification of bacteria, from basic based on visual recognition of the shape and / or the symptoms generated each bacteria (at least the best known) to in-depth studies on the morphology, reactions chemical and even the DNA of bacteria, to know more you can see.

National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov/
High Quality Ribosomal RNA databases, SILVA: http://www.arb-silva.de/browser/
Ribosomal Database Project: http://rdp.cme.msu.edu/index.jsp

By the nature of our system, we chose a simple and common test in microbiology classes, is recognized for its quality and its ease of implementation. It is based on the chemical reaction that has the bacteria in different culture media, one of the most popular is “Test EnteroPluri” developed by the Italian company Liofilchem (http://www.liofilchem.net/ ).

In the brochure Liofilchem, we see the nine simple steps to identify the bacteria.

More information:
http://www.liofilchem.net/en/pdf/enteropluritest.pdf
http://www.liofilchem.net/login/pd/pi/78618_PI.pdf

We appreciate all the support and patience of team-DICSA Microbiology, UDEM.

On the other hand on the open hardware, find the color recognition technology, is a fabulous TCS34725 Adafruit PCB, you can see more information on Input Devices:

http://fabacademy.org/archives/2015/sa/students/meneses.fernando/10_input%20devices.html

With these references, we can see that appears possible to develop a device that can recognize changes color by placing the bacteria in different culture media, and thereby identify the bacteria.

Materials and Components

what materials and components will be required?

RGBacteria functions open as a peripheral device, so it will be necessary to control a computer, and by now consist of:

  1. hardware:
    OpenRGBacteria-Petri
    OpenRGBacteria-PCB
  2. software:
    OpenRGBacteria-Code
    OpenRGBacteria-Interface
    OpenRGBacteria-Database

Origin of components

where will they come from?

1. hardware:
1.1 OpenRGBacteria-Petri
based on_
->> molding and casting: http://academy.cba.mit.edu/classes/molding_casting/index.html

1.2 OpenRGBacteria-PCB
based on_
->> FabKit: http://fab.cba.mit.edu/content/projects/fabkit/
->> hello.RGB: http://academy.cba.mit.edu/classes/output_devices/index.html
->> TCS34725 Adafruit: https://github.com/adafruit/Adafruit_TCS34725
->> hello.FTDI: http://academy.cba.mit.edu/classes/embedded_programming/index.html

software:
2.1 OpenRGBacteria-Code
based on_
->> FabKit: http://academy.cba.mit.edu/classes/embedded_programming/index.html
->> hello.RGB: http://academy.cba.mit.edu/classes/output_devices/index.html
->> TCS34725 Adafruit: https://github.com/adafruit/Adafruit_TCS34725
->> hello.FTDI: http://academy.cba.mit.edu/classes/embedded_programming/index.html
->> IDE Arduino: http://www.arduino.cc/

2.2 OpenRGBacteria-Interface
developed in_
->> Processing: https://processing.org/

2.3 OpenRGBacteria-Database
developed in_
->> SQL-BezierSQLib: https://github.com/fjenett/sql-library-processing

 

Cost of the first prototype

how much will it cost?

This is an initial exercise of the cost of the first test prototype, the ideal is that the end device is inexpensive.

part

component

description

process

quantity

cost / unit

cost/ pesos MXN
OpenRGBacteria-Petri Molde en negativo Cera azul Fresado SRM-20

1pz

$60.00

$60.00
Molde Silicon de caucho Vaciado al vacío

1pz

$50.00

$50.00
Pieza(de 1 a 100) Resina Vaciado al vacío

1pz

$30.00

$30.00
OpenRGBacteria-PCB PCB PCB FR1 Fresado SRM-20

1pz

$10.00

$10.00
Componente Capacitor 0.1uf Soldadura superficial

1pz

$5.44

$5.44
Componente Capacitor 1uf, np Soldadura superficial

2pz

$3.91

$7.82
Componente Capacitor 0.1uf, np Soldadura superficial

13pz

$5.44

$70.72
Componente Capacitor 10uf, np Soldadura superficial

25pz

$6.29

$157.25
Componente ATMega 328p Soldadura superficial

1pz

$57.63

$57.63
Componente AtTiny 44 Soldadura superficial

1pz

$20.00

$20.00
Componente LED YGC 1206smd Soldadura superficial

1pz

$5.95

$5.95
Componente LED EV45-21 Soldadura superficial

12pz

$6,24

$74.88
Componente Resistencia 10k Soldadura superficial

62pz

$1.53

$94.86
Componente Resistencia 499k Soldadura superficial

1pz

$1.53

$1.53
Componente Resistencia TBD Soldadura superficial

12pz

$3.24

$38.88
Componente Switch 6mm Soldadura superficial

1pz

$19.89

$19.89
Componente Pin header 1×6 Soldadura superficial

1pz

$19.72

$19.72
Componente Pin header 2×3 Soldadura superficial

1pz

$19.72

$19.72
Componente Resonador 16Mhz Soldadura superficial

1pz

$5.61

$5.61
Componente Resonador 20Mhz Soldadura superficial

1pz

$8.52

$8.52
Componente Mosfet BSS138 Soldadura superficial

36pz

$6.00

$216.00
Componente TCS34725 Soldadura superficial

12pz

$33.24

$398.88
Componente RT9193 Soldadura superficial

12pz

$6.00

$72.00

Total

$1445.30 MXN

$ 96.32 USD

 

Scope of development

what parts and systems will be made?

hardware:
OpenRGBacteria-Petri                / designed and manufactured in FabLab Monterrey
OpenRGBacteria-PCB                /designed and manufactured in FabLab Monterrey
software:
OpenRGBacteria-Code               /designed in FabLab Monterrey
OpenRGBacteria-Interface       /designed in FabLab Monterrey
OpenRGBacteria-Database       /data not included

 

Processes used

what processes will be used?

hardware:
OpenRGBacteria-Petri              /Molding and Casting
OpenRGBacteria-PCB               /Electronics Production
software:
OpenRGBacteria-Code             /Embedded Programming
OpenRGBacteria-Interface      /Interface and Application Programming
OpenRGBacteria-Database     /Interface and Application Programming

 

Pending to do

what tasks need to be completed?

activity

technological base

design

manufacturing

testing

version 0.0
OpenRGBacteria-Petri 100%

80%

30%
OpenRGBacteria-PCB 100%

50%
OpenRGBacteria-Code  100%

50%
OpenRGBacteria-Interface  100%

80%

50%
OpenRGBacteria-Database  100%

50%

 

what questions need to be answered?

Work schedule

what is the schedule?

activity

May 13

May 20

May 27

Jun 3

Jun 10
Project planning applications and implications
OpenRGBacteria-Petri

design

manufacturing
OpenRGBacteria-PCB

design

manufacturing
OpenRGBacteria-Code

design

design
OpenRGBacteria-Interface

design

design
OpenRGBacteria-Database

design

design
Project presentation

Version 0.0

 

Valuation indicators

how will it be evaluated?

Finally, the device has to be reviewed by the two main indicators:

– Quality in recognizing bacteria

-Accessibility and low cost

part

component

description

process

quantity

cost / unit

cost/ pesos MXN
OpenRGBacteria-Petri Molde en negativo Cera azul Fresado SRM-20

1pz

$10.00

$10.00
Molde Silicon de caucho Vaciado al vacío

1pz

$20.00

$20.00
Pieza(de 1 a 100) Resina Vaciado al vacío

1pz

$10.00

$10.00
OpenRGBacteria-PCB PCB PCB FR1 Fresado SRM-20

1pz

$10.00

$10.00
Componente Capacitor 0.1uf Soldadura superficial

1pz

$5.44

$5.44
Componente Capacitor 1uf, np Soldadura superficial

2pz

$3.91

$7.82
Componente Capacitor 0.1uf, np Soldadura superficial

2pz

$5.44

$10.88
Componente Capacitor 10uf, np Soldadura superficial

3pz

$6.29

$18.87
Componente ATMega 328p Soldadura superficial

1pz

$57.63

$57.63
Componente AtTiny 44 Soldadura superficial

1pz

$20.00

$20.00
Componente LED YGC 1206smd Soldadura superficial

1pz

$5.95

$5.95
Componente LED EV45-21 Soldadura superficial

1pz

$6,24

$6.24
Componente Resistencia 10k Soldadura superficial

7pz

$1.53

$10.71
Componente Resistencia 499k Soldadura superficial

1pz

$1.53

$1.53
Componente Resistencia TBD Soldadura superficial

1pz

$3.24

$3.24
Componente Switch 6mm Soldadura superficial

1pz

$19.89

$19.89
Componente Pin header 1×6 Soldadura superficial

1pz

$19.72

$19.72
Componente Pin header 2×3 Soldadura superficial

1pz

$19.72

$19.72
Componente Resonador 16Mhz Soldadura superficial

1pz

$5.61

$5.61
Componente Resonador 20Mhz Soldadura superficial

1pz

$8.52

$8.52
Componente Mosfet BSS138 Soldadura superficial

3pz

$6.00

$18.00
Componente TCS34725 Soldadura superficial

1pz

$33.24

$33.24
Componente RT9193 Soldadura superficial

1pz

$6.00

$6.00

Total

$320.49 MXN

$ 20.60 USD

_conclusions

To develop a device with 12 sensors is very expensive, and in practice with a sensor device and add usability work days’ just as well that this will develop low-cost version of the device, only a color sensor, here the table of only one sensor.

_

Original source: http://academy.cba.mit.edu/classes/applications_implications/index.html

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Contact:  fernando.meneses@udem.edu / fernandomeneses@nodolab.com /  f  /  in  /  g+ / b / v / mx / w